Regulatory

Part:BBa_K3789004:Design

Designed by: MEI YING YI   Group: iGEM21_UM_Macau   (2021-09-26)


pGK1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pGK1 is a common promoter for expression systems. For SOD and PNP, sustained expression throughout the whole fermentation process before the removal of yeast is beneficial, so the non-conditional-specific pGK1 promoter can be used.



Source

The sequence we used comes from Saccharomyces cerevisiae S288c


References